This GIS dataset contains flying bird data from field work in the Windmill Islands by Jan van Franeker at Ardery Island and Odbert Island. Polygon data represents nesting areas. Point data represents nest locations on Ardery Island.

This GIS dataset contains bird data from 1998/99 field work in the Windmill Islands by Jonny Stark and Jeroen Creuwels. The locations are Frazier Islands, Ardery Island and Casey station. Polygon data represents the extents of flying bird nesting areas and adelie penguin colonies. Point data represents flying bird nest locations.

Aerial photography (Linhof) of penguin colonies was acquired over the Windmill Islands (Eric Woehler). The penguin colonies were traced, then digitised (John Cox), and saved as DXF-files. Using the ArcView extension 'Register and Transform' (Tom Velthuis), The DXF-files were brought into a GIS and transformed to the appropriate islands. Data conforms to SCAR Feature Catalogue which can be searched (refer to link below).

This GIS dataset is the result of field work in the Windmill Islands by Eric Woehler. The locations are Frazier Islands, Shirley Island, Ford Island and Casey station. Polygon data represents the extents of flying bird nesting areas and adelie penguin colonies. Point data represents nest locations.

Ten sediment cores were collected from 3 marine bays in the Windmill Islands. Two cores were collected in Sparkes Bay, one in Shannon Bay, and seven in Brown Bay. Only diatom data are presented here, however Pb210 and metal analyses have also been undertaken - contact Ian Snape (ian.snape@aad.gov.au) for more information regarding this. The diatom spreadsheet (diatom_data) lists the relative abundance of benthic species. The abbreviation used to identify species are explained in the separate file called sp_list. Each core has been saved as a separate file. The STE cores were collected from within a couple of meters of each other. These cores were collected in close proximity to a tip site at one end of Brown Bay. BBMid was collected from the middle of the bay, while BB Outer 1 and 2 were collected from the outer regions of this bay, and thus represent the greatest distance from the tip site. Unless otherwise stated, the lowest number within each core represents the youngest sample. This work was completed as part of ASAC project 1130 (ASAC_1130) and project 2201 (ASAC_2201). Public summary from project 1130: Algal mats grow on sea floor in most shallow marine environments. They are thought to contribute more than half of the total primary production in many of these areas, making them a critical food source for invertebrates and some fish. We will establish how important they are in Antarctic marine environments and determine the effects of local sewerage and tip site pollution. We will also investigate the impact on the algal mats of the additional UV radiation which results from the ozone hole. Public summary from project 2201: As a signatory to the Protocol on Environmental Protection to the Antarctic Treaty Australia is committed to comprehensive protection of the Antarctic environment. This protocol requires that activities in the Antarctic shall be planned and conducted on the basis of information sufficient to make prior assessments of, and informed judgements about, their possible impacts on the Antarctic environment. Most of our activities in the Antarctic occur along the narrow fringe of ice-free rock adjacent to the sea and many of our activities have the potential to cause environmental harm to marine life. The Antarctic seas support the most complex and biologically diverse plant and animal communities of the region. However, very little is known about them and there is certainly not sufficient known to make informed judgements about possible environmental impacts. The animals and plants of the sea-bed are widely accepted as being the most appropriate part of the marine ecosystem for indicating disturbance caused by local sources. Attached sea-bed organisms have a fixed spatial relationship with a given place so they must either endure conditions or die. Once lost from a site recolonisation takes some time, as a consequence the structure of sea-bed communities reflect not only present conditions but they can also integrate conditions in the past. In contrast, fish and planktonic organisms can move freely so their site of capture does not indicate a long residence time at that location. Because sea-bed communities are particularly diverse they contain species with widely differing life strategies, as a result different species can have very different levels of tolerance to stress; this leads to a range of subtle changes in community structure as a response to gradually increasing disturbance, rather than an all or nothing response. This project will examine sea-bed communities near our stations to determine how seriously they are affected by human activities. This information will be used to set priorities for improving operational procedures to reduce the risk of further environmental damage. The fields in this dataset are: Species Site Abundance Benthic

Distribution, abundance and dates of relict Adelie Penguin colonies in the Australian Antarctic Territory (AAT). Current mapping efforts have focused on the Windmill Islands in preparation for a PhD study to commence in 2004/05 with the two investigators. The planned PhD study will work at either the Windmill Islands or the Vestfold Hills. This project integrates ASAC projects 1219 and 1322 (ASAC_1219, ASAC_1322). The fields in the excel spreadsheet are: Radiocarbon Samples Isotope Samples Site - list of precise locations provided in the downloadable paper Level - horizontal stratum (depth), given in 5cm blocks Species Material Weight (g) Notes Lab no. Uncorrected Date (BP) - (day) Standard Deviation Delta R - range of corrected date for sample, 2 standard deviations either side of the mean Mean - estimated mean of sample date See the paper included in the download file for further information.

A hierarchical, 3-level, nested design was used. The highest hierarchical level consisted of six locations. Two of these locations, Brown Bay and Shannon Bay, have been contaminated with heavy metals (Stark et al., 2003; Snape et al., 2001); Brown Bay has also been contaminated with petroleum hydrocarbons (Snape et al., 2001). The remaining four locations are more distant from Casey Station and were used as control locations. These locations were Denison Island, Odbert Island, O'Brien Bay and Sparkes Bay. A full description of these sites is given below. Within each location two sites were selected approximately 100 m apart. Within each site, two plots were sampled (~ 10 m apart). Although the sampling program had been designed for four replicates within each plot, the patchy distribution of bottom sediments in the Windmill Islands restricted this to two replicate samples (~ 1 m apart) per plot. Samples were collected using an Eckman grab sampler, deployed from a boat. To minimise the potential influence of water depth, all samples were collected from 8 m water depth. Samples were collected within a three day period in early February when no sea-ice was present. Diatom data are presented as the relative abundances of benthic species. Samples are identified xyz where x = first initial of sample location (or first 2 initials where 2 locations start with the same letter), y = plot number (plots 1 and 2 represent site 1, while plots 3 and 4 are from site 2), and z = replicate number (a or b). Abbreviations used for species are shown in the separate file sp_list. This work was completed as part of ASAC project 1130 (ASAC_1130) and project 2201 (ASAC_2201). Public summary from project 1130: Algal mats grow on sea floor in most shallow marine environments. They are thought to contribute more than half of the total primary production in many of these areas, making them a critical food source for invertebrates and some fish. We will establish how important they are in Antarctic marine environments and determine the effects of local sewerage and tip site pollution. We will also investigate the impact on the algal mats of the additional UV radiation which results from the ozone hole. Public summary from project 2201: As a signatory to the Protocol on Environmental Protection to the Antarctic Treaty Australia is committed to comprehensive protection of the Antarctic environment. This protocol requires that activities in the Antarctic shall be planned and conducted on the basis of information sufficient to make prior assessments of, and informed judgements about, their possible impacts on the Antarctic environment. Most of our activities in the Antarctic occur along the narrow fringe of ice-free rock adjacent to the sea and many of our activities have the potential to cause environmental harm to marine life. The Antarctic seas support the most complex and biologically diverse plant and animal communities of the region. However, very little is known about them and there is certainly not sufficient known to make informed judgements about possible environmental impacts The animals and plants of the sea-bed are widely accepted as being the most appropriate part of the marine ecosystem for indicating disturbance caused by local sources. Attached sea-bed organisms have a fixed spatial relationship with a given place so they must either endure conditions or die. Once lost from a site recolonisation takes some time, as a consequence the structure of sea-bed communities reflect not only present conditions but they can also integrate conditions in the past. In contrast, fish and planktonic organisms can move freely so their site of capture does not indicate a long residence time at that location. Because sea-bed communities are particularly diverse they contain species with widely differing life strategies, as a result different species can have very different levels of tolerance to stress; this leads to a range of subtle changes in community structure as a response to gradually increasing disturbance, rather than an all or nothing response. This project will examine sea-bed communities near our stations to determine how seriously they are affected by human activities. This information will be used to set priorities for improving operational procedures to reduce the risk of further environmental damage. The fields in this dataset are: Species Site Abundance Benthic

Sediment samples were collected from four locations within the Windmill Islands (Cloyd Island, Odbert Island, Shannon Bay and Brown Bay). Within each location three parallel transects were created, with samples taken at set depths along each transect. At the time of collection, both surface and benthic irradiance levels were measured, and the % of surface irradiance that reached the sediment-water interface was calculated. Samples were analysed for benthic diatom abundances (expressed as relative abundances), and grain-size (expressed as % of total weight). The diatom spreadsheet (diatom_data)lists the relative abundance of benthic species. The abbreviation used to identify species are explained in the separate file called sp_list. Samples are identified XTYZ where X is the first letter of the location, Y indicates the sampling position along the transect and z indicates the transect (a, b or c). The benthic sheet is the relative abundances of benthic species. The greater than 2% sheet lists all the species that reach abundances greater than2% in at least 1 sample. The table sheet has the same info as greater than 2% but arranged by the individual locations. In this sheet (tables), measurements in m represent the depth of the water column overlying the position where the sediment samples were collected. (ie it was at different locations, not different water depths in the one spot). Sampling positions reflect increasing depth. At Brown Bay and Odbert Island, sediment samples were collected below water columns/water depths of 1, 2, 4, 8 and 12 m. At Cloyd Island, samples were collected from 4,6,8 and 12 m water depths. At Shannon Bay samples were collected from 2, 4, 8, and 12 m water depths. Details of the environmental parameters examined (grainsize and light) are given in the file labelled 'env_data' This work was completed as part of ASAC project 1130 (ASAC_1130). Public summary from project 1130: Algal mats grow on sea floor in most shallow marine environments. They are thought to contribute more than half of the total primary production in many of these areas, making them a critical food source for invertebrates and some fish. We will establish how important they are in Antarctic marine environments and determine the effects of local sewerage and tip site pollution. We will also investigate the impact on the algal mats of the additional UV radiation which results from the ozone hole. The fields in this dataset are: Diatom Spreadsheet Species Site Location Transect Depth (m) Environmental Data Spreadsheet Location Transect Depth (m) Grain size Gravel Sand Mud Light

A collection of about 20 isolates of Antarctic microalgae from the Windmill Islands region, around Casey Station has been established in the University of Malaya Algae Culture Collection (UMACC). The Antarctic microalgae in the collection includes Chlamydomonas, Chlorella, Stichococcus, Navicula. Ulothrix and Chlorosarcina. Comparative studies on the effect of global warming and UVR stress on these Antarctic microalgae and the tropical collection are being conducted. From the abstract of one of the referenced papers: The growth, biochemical composition and fatty acid profiles of six Antarctic microalgae cultured at different temperatures, ranging from 4, 6, 9, 14, 20 to 30 degrees C, were compared. The algae were isolated from seawater, freshwater, soil and snow samples collected during our recent expeditions to Casey, Antarctica, and are currently deposited in the University of Malaya Algae Culture Collection (UMACC). The algae chosen for the study were Chlamydomonas UMACC 229, Chlorella UMACC 234, Chlorella UMACC 237, Klebsormidium UMACC 227, Navicula UMAC 231 and Stichococcus UMACC 238. All the isolates could grow at temperatures up to 20 degrees C; three isolates, namely Navicula UMACC 231 and the two Chlorella isolates (UMACC 234 and UMACC 237) grew even at 30 degrees C. Both Chlorella UMACC 234 and Stichococcus UMAC 238 had broad optimal temperatures for growth, ranging from 6 to 20 degrees C (growth rate = 0.19 - 0.22 per day) and 4 to 14 degrees C (growth rate = 0.13 - 0.16 per day), respectively. In constrast, optimal growth temperatures for Navicula UMACC 231 and Chlamydomonas UMACC 229 were 4 degrees C (growth rate = 0.34 per day) and 6 to 9 degrees C (growth rate = 0.39 - 0.40 per day), respectively. The protein content of the Antarctic algae was markedly affected by culture temperature. All except Navicula UMACC 231 and Stichococcus UMACC contained higher amount of proteins when grown at low temperatures (6-9 degrees C). The percentage of PUFA, especially 20:5 in Navicula UMACC 231 decreased with increasing culture temperature. However, the percentages of unsaturated fatty acids did not show consistent trend with culture temperature for the other algae studied. There are three spreadsheets available in the download file. ASAC_2590 - provides detail about where each species of algae was collected from. ASAC_2590a - provides data from Teoh Ming-Li et al (2004) ASAC_2590b - provides data from Wong Chiew-Yen et al (2004) The fields in this dataset are: Isolate Culture Collection number Origin (Location) Fatty acids saturated fatty acids polyunsaturated fatty acids monounsaturated fatty acids Temperature growth rate PAR UVB

This metadata record contains the results from bioassays conducted to show the response of an Antarctic nemertean Antarctonemertes unilineata to contamination from combinations of Special Antarctic Blend (SAB) diesel, Marine Gas Oil (MGO) and Intermediate Fuel Oil (IFO 180), chemically dispersed with fuel dispersants Ardrox 6120, Slickgone LTSW and Slickgone NS. Note that the corresponding PhD thesis chapter refers to the species as Antarctonemertes sp., prior to being named Antarctonemertes unilineata in 2018. Experiments using SAB, MGO and IFO 180 with the dispersant Ardrox 6120, including fuel only and dispersant only treatments were conducted at Casey station. Experiments involving IFO 180 and the fuel dispersants Slickgone LTSW and Slickgone NS were conducted at the Antarctic Division’s Marine Research Facility quarantine labs. All experimental procedures, including test mix preparation and bioassays were conducted at 0 plus or minus 1 degree C. Water accommodated fractions (WAF; fuel mixed in water) and chemically enhanced water accommodated fractions (CEWAF) were made according to the specifications of Singer, Aurand et al. (2000), Barron and Ka’aihue (2003) and Kotzakoulakis (unpublished at time of writing). Dispersant only mixes were also made using filtered seawater (FSW) and dispersant volumes proportional to those used for CEWAF production. WAF was made using a loading ratio of 1: 25 (v/v) fuel to FSW, CEWAF was prepared using 1:100 (v/v) fuel to FSW ratio, and 1: 20 (v/v) dispersant to fuel ratio. Following the 48 h preparation time, the seawater WAF components of the mix were drained from the bottom of aspirator bottles and serially diluted. WAF treatment concentrations were 100%, 50%, 20% and 10%, CEWAF and dispersant only concentrations were 10%, 5%, 1% and 0.1%. Treatment solutions were replenished every four days to simulate a repeated pulse exposure to contaminants and to replace hydrocarbons lost through evaporation and adsorption and to maintain water quality parameters. WAF, CEWAF and dispersant only test solutions were remade every four days using identical methods. Tests were done in temperature-controlled cabinets set to 0 plus or minus 1 degree C following a 6 h light to 18 h dark photoperiod. Beakers were left uncovered to allow for the natural evaporation of lighter hydrocarbon components to reflect real fuel spill conditions. Experiments ran for 24 d except for the Ardrox 6120 only experiment, which ran for 16 d due to high mortality in this treatment. Sublethal and lethal endpoints were assessed at 1, 2, 4, 7, 8, 12, 14, 16, 20 and 24 d observations. Aliquot water samples for analysis of total hydrocarbon content (THC) were taken for initial and final test concentrations, and before and after each four-day water change, to obtain accurate profiles of hydrocarbon loss over the test period. Duplicate samples were taken for every treatment concentration and extracted with dichloromethane, spiked with an internal standard of 1-bromoeicosane and cyclooctane. Samples were analysed using gas chromatography with flame ionization detection (GC-FID) and gas chromatography mass spectrometry (GC-MS). Average THC concentrations for the duration of the experiment were obtained by integrating the measured concentrations to which animals were exposed following the methods of Brown et al. (2016) and Payne et al. (2014). This data submission includes one file detailing the TPH experiment analyses and one detailing the bioassay tests and results. The thesis that relates to this work is available from: https://epubs.scu.edu.au/theses/533/